IGEM:Cambridge/2008/Electric Output: Difference between revisions
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=Current Progress= | =Current Progress= | ||
[ | [[IGEM:Cambridge/2008/Notebook/Voltage| Progress Notebook]] | ||
=Next Steps= | =Next Steps= | ||
Latest revision as of 09:09, 17 July 2008
Aim
To create a system which responds to ligand binding with a detectable voltage caused by a K+ flux.
Background
Current Progress
Next Steps
Characterise promoters
1. Simulate and design ‘reporter plasmids’ with correct biobricks restriction enzyme sites
2. For each strain: 2 plasmid backbones
- PSC101 (A) with OsmY (promoter) + RFP + Stop
- Another (B), not yet defined, with Bba_J23100 + YFP + Stop
- Tests for each strain : plasmid A, plasmid B, plasmid A + plasmid B (so 15 tests)
3. Quantify transformation efficiency (colony counter)
4. Quantify promoter strength (light intensity, expression levels)
Useful Links
Literature
The Kdp-ATPase system and its regulation
Potential Chassis: Strain JW1242-1 Strain JW0710-1
