IGEM:Cambridge/2008/Electric Output/Meeting with Julia Davies: Difference between revisions
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(New page: -Discussed feasibility -Osmotic problems with ion sequestration, possible solution - "inert" sugars such as sorbitol and manitol -Glutamate/glutamine specificity problems, may need gluta...) |
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Revision as of 09:04, 17 July 2008
-Discussed feasibility
-Osmotic problems with ion sequestration, possible solution - "inert" sugars such as sorbitol and manitol
-Glutamate/glutamine specificity problems, may need glutamine- as well as glutamate-free growth medium
-Need to test efficacy of voltage creation over range of osmolarities, use flame photometer to plot total K+ as fn of growth curve
-Discussed Kch- mutants, generally benign
-Potential PD measurement methods: high resistance input voltmeter to minimise noise, glass capillary microelectrode, oxygen electrode
-Measurement issue of cells acting as charged particles, neutralising charge around electrode
