IGEM:Cambridge/2008/Flame Photometer: Difference between revisions

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=Set up=


:*Make sure discharge pipe (on righthand side) is placed in something to collect discharge, and valve is not completely shut.
:*Check clear tube is connected to compressor OUTLET, and securely attached.
:*Turn on compressor.
:*Gauge on back of photometer should read 12psi.
:*Check gas pipe is attached at tap.
:*Turn on gas.
:*Wait 1 minute.
:*Turn on photometer. It should click, and orange "flame on" light should come on.
:*Place beaker of distilled water in the photometer tray, and make sure thin tube reaches water.
:*Wait 15 minutes before taking any readings.
:*Use "blank" dial to adjust reading to 0 for distilled water.
=Taking readings=
:*Place thin tube into sample, far in, as the liquid will be taken up quite rapidly.
:*Wait for reading to stabilise (reading may continue to fluctuate at high values) and take reading.
:*Place tube in distilled water beaker between each sample, waiting until reading returns to 0.
=In case of compressor blow-out=
:*Turn off gas at tap.
:*Turn off photometer.
:*Turn off compressor.
:*Redo set up steps, with 15 minute waiting time reduced to 5, as the equipment will already be almost up to temperature.

Latest revision as of 08:16, 28 July 2008