IGEM:Cambridge/2008/Notebook/Bacillus

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==Description/Abstract==
 
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* Place short description of project or notes regarding this project
 
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* Lorem ipsum dolor sit amet, consectetuer adipiscing elit. Donec porta commodo tellus. Nam a est eget libero mollis tincidunt. Aliquam purus. Quisque nulla ligula, facilisis in, pulvinar sed, molestie a, quam. Vestibulum at pede. In in sem eget odio eleifend placerat. Phasellus ultricies felis quis sapien. Etiam molestie volutpat quam. Praesent pulvinar scelerisque mi. Nam mi urna, fringilla eu, mattis sed, venenatis id, nunc. Maecenas velit eros, congue ut, placerat in, ornare vel, sem. Aenean porta enim sit amet felis gravida posuere. Phasellus faucibus nibh et orci.
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=Links=
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* Chris French's [http://openwetware.org/wiki/Cfrench:BacTrans1 protocol on transforming B. subtilis]
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* We have last year's protocol; not sure if it's trustworthy, need to compare with online resources
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* [[IGEM:Cambridge/2008/Turing_Pattern_Formation/BCSG_Transformation_Protocol|Protocol from BCSG catalog]]
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** used in Cornell bacteriology course, probably should start here
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==Notes==
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[http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T30-3VCK5Y0-3&_user=1495569&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000053194&_version=1&_urlVersion=0&_userid=1495569&md5=847137ffc5cf267e12b5625aaadecb0e B. Subtilis Transformation Protcol - Electroporation]
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* Place some notes here for visitors
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* Example: Next team meeting on Tuesday, June 18th! Be there!
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[http://aem.highwire.org/cgi/content/abstract/71/12/8818 Bongers et al. … of a Subtilin-Regulated Expression System in Bacillus subtilis: Strict Control of Gene Expression …. Applied and Environmental Microbiology (2005)]
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* SURE: handy expression system for B. subtilis
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* What about feedback/regulation effects of subtilin on the cell
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[http://lib.bioinfo.pl/pmid:18261893 Expression and characterization of aiiA gene from Bacillus subtilis BS-1.]
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* (Some strains of) Bacillus subtilis produces a gene called aiiA that degrades AHL molecules from gram-negative bacteria
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* this is biobricked
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== Links to Cambridge 2007 Wiki ==
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*[http://www.ccbi.cam.ac.uk/iGEM2007/index.php/Bacillus_subtilis_SynBio_chassis B. subtilis group]
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*[http://www.ccbi.cam.ac.uk/iGEM2007/index.php/Detailed_background_information_-_B._subtilis B. subtilis group: more info]
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Revision as of 09:30, 2 September 2008



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B. Subtilis Transformation Protcol - Electroporation

Bongers et al. … of a Subtilin-Regulated Expression System in Bacillus subtilis: Strict Control of Gene Expression …. Applied and Environmental Microbiology (2005)

  • SURE: handy expression system for B. subtilis
  • What about feedback/regulation effects of subtilin on the cell

Expression and characterization of aiiA gene from Bacillus subtilis BS-1.

  • (Some strains of) Bacillus subtilis produces a gene called aiiA that degrades AHL molecules from gram-negative bacteria
  • this is biobricked

Links to Cambridge 2007 Wiki



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