Testing B.subtilis promoters and RBSs

We are seeking to test the expression strength and response to inducers in several promoters in B.subtilis.

Links

• Chris French's protocol on transforming B. subtilis
• We have last year's protocol; not sure if it's trustworthy, need to compare with online resources
• Protocol from BCSG catalog
  • used in Cornell bacteriology course, probably should start here

B. Subtilis Transformation Protcol - Electroporation

Bongers et al. … of a Subtilin-Regulated Expression System in Bacillus subtilis: Strict Control of Gene Expression …. Applied and Environmental Microbiology (2005)

• SURE: handy expression system for B. subtilis
• What about feedback/regulation effects of subtilin on the cell

Expression and characterization of aiiA gene from Bacillus subtilis BS-1.

• (Some strains of) Bacillus subtilis produces a gene called aiiA that degrades AHL molecules from gram-negative bacteria
• this is biobricked

Links to Cambridge 2007 Wiki

• B. subtilis group
• B. subtilis group: more info