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Testing B.subtilis promoters and RBSs
We are seeking to test the expression strength and response to inducers in several promoters in B.subtilis, in combination with different Bacillus-specific ribosome binding sites.
B. subtilis Promoter Testing with Beta-galactosidase Assay
We are aiming to characterize four different Bacillus subtilis promoters using the beta-galactosidase assay. (Protocol for beta-galactosidase assay as described here
B. subtilis Promoters to be Tested:
Links to Cambridge 2007 Wiki