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Creating new Biobrick-compatible B.subtilis vectors
We are using the InFusion PCR method from ClonTech to create new Biobrick-compatible integrative and episomal vectors. These vectors will allow us to insert Biobricks into the Bacillus subtilis genome at the AmyE locus and as a 3-5 copy plasmid that will replicate autonomously in Bacillus.
Testing B.subtilis promoters and RBSs
We are seeking to test the expression strength and response to inducers in several promoters in B.subtilis, in combination with different Bacillus-specific ribosome binding sites.
B. subtilis Promoter Testing with Beta-galactosidase Assay
We are aiming to characterize four different Bacillus subtilis promoters using the beta-galactosidase assay. (Protocol for beta-galactosidase assay as described here
B. subtilis Promoters to be Tested:
Links to Cambridge 2007 Wiki