IGEM:Cambridge/2008/Notebook/Bacillus/2008/07/22: Difference between revisions

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{{Cambridge08a}}
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|style="background-color: #EEE"|[[Image:igem-logo-150px.png|150px]]<span style="font-size:22px;"> Working with Bacillus</span>
|style="background-color: #444444"|[[Image:Bacillus_button.gif|200px|center]]
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Revision as of 04:08, 5 September 2008

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We have 4 tubes from last year. These strains are frozen in glycerol.

  • PNZ8901 plasmid in E.coli MC1061 Strain
    • Chloroamphenicol resistant
    • "sure" vector
    • does not integrate
  • B. subtilis strain 1A1
    • No resistance
    • Same as strain 168 but tryptophan deficient
  • E.coli strain MC1061, no plasmid
  • PP182 plasmid in E.coli strain DH5alpha
    • Ampicillin resistance
    • integrates


Checking antibiotic resistance

Purpose : Grow each of them on a plate to test their antibiotic resistance

Protocol

  • Warm up frozen tubes
  • Take 15uL of each and put it on plates without antibiotic
  • Incubate at 37°C
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