IGEM:Cambridge/2008/Notebook/Bacillus/2008/08/01

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Wet Work

  • Grow received vectors
Vectors AmpR (μg/μL) CmR (μg/μL) KanR (μg/μL)
ECE112 100 0 0
ECE147 50 0 0
ECE149 50 0 0
ECE150 50 0 0
ECE151 50 0 0
ECE153 100 0 0
ECE162 100 0 0
ECE165 100 10 0
ECE166 100 10 0
ECE171 50 0 10
ECE172 100 10 0
ECE176 100 5 0

- Preparation of Agar plates

We have bottles of 200mL.

Type Amp (μL) Cm (μL) Kan (μL)
Amp100 200 0 0
Amp50 100 0 0
Amp100 + Cm10 200 57.1 0
Amp100 + Cm5 200 28.6 0
Amp50 + Kan10 50 0 80


- Preparation of LB tubes

We prepare 10mL of LB in which we add a single colony.


Type Amp (μL) Cm (μL) Kan (μL)
Amp100 10 0 0
Amp50 5 0 0
Amp100 + Cm10 10 2.9 0
Amp100 + Cm5 10 1.4 0
Amp50 + Kan10 5 0 4



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