IGEM:Cambridge/2008/Notebook/Bacillus/2008/08/26: Difference between revisions
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(Autocreate 2008/08/26 Entry for IGEM:Cambridge/2008/Notebook/Bacillus) |
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== | ==Xylose test== | ||
* | * for ECE153 | ||
- Prepare 4 tubes with 10mL LB and Spc50, inoculate each tube with a colony from IA751 + ECE153 | |||
==Starch plate== | |||
- prepare some blank agar plates | |||
- add 1g of starch into 100mL of soft agar, mix, boil | |||
- put a thin layer of soft agar with starch | |||
- plate with IA751, IA771, IA751+ECE153 and IA751 (control plate, to see if we mixed some plates) | |||
- Incubate at 37°C | |||
==Ery plate== | |||
- Plate IA751, IA771, IA751 (control plate) and IA751+ECE153 | |||
Revision as of 09:02, 5 September 2008
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Xylose test
- Prepare 4 tubes with 10mL LB and Spc50, inoculate each tube with a colony from IA751 + ECE153 Starch plate- prepare some blank agar plates - add 1g of starch into 100mL of soft agar, mix, boil - put a thin layer of soft agar with starch - plate with IA751, IA771, IA751+ECE153 and IA751 (control plate, to see if we mixed some plates) - Incubate at 37°C Ery plate- Plate IA751, IA771, IA751 (control plate) and IA751+ECE153
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