IGEM:Cambridge/2008/Notebook/Bacillus/2008/09/08: Difference between revisions
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- Add 12μL of SDW, 5 μL of MM, 1μL + 1μL of each primers (for AmyE back and AmyE front) and 1μL of ECE112 | - Add 12μL of SDW, 5 μL of MM, 1μL + 1μL of each primers (for AmyE back and AmyE front) and 1μL of ECE112 | ||
* Lane1 : HyperladderI | |||
* Lane4 : AmyE front | |||
* Lane5 : AmyE front | |||
* Lane6 : AmyE back | |||
* Lane7 : AmyE back | |||
[[Image:gelPCR08/09.gif|100px|center]] | |||
* Results : good band for AmyE front, very low band for AmyE back | |||
<!-- ## Do not edit below this line unless you know what you are doing. ## --> | <!-- ## Do not edit below this line unless you know what you are doing. ## --> |
Revision as of 07:06, 9 September 2008
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Prepare chromosomal DNA extraction from Bacillus- Grow 2 tubes into LB for
PCR AmyE from ECE112- Add 12μL of SDW, 5 μL of MM, 1μL + 1μL of each primers (for AmyE back and AmyE front) and 1μL of ECE112
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