IGEM:Cambridge/2008/Notebook/Bacillus/2008/09/08: Difference between revisions
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* Lane7 : AmyE back | * Lane7 : AmyE back | ||
[[Image:gelsdgsdg.gif| | [[Image:gelsdgsdg.gif|300px|center]] | ||
* Results : good band for AmyE front, very low band for AmyE back | * Results : good band for AmyE front, very low band for AmyE back |
Revision as of 07:20, 9 September 2008
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Prepare chromosomal DNA extraction from Bacillus- Grow 2 tubes into LB for
PCR AmyE from ECE112- Add 12μL of SDW, 5 μL of MM, 1μL + 1μL of each primers (for AmyE back and AmyE front) and 1μL of ECE112
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