IGEM:Cambridge/2008/Notebook/Bacillus/2008/09/09: Difference between revisions
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* Lane2 : AmyE Back | * Lane2 : AmyE Back | ||
* Lane3 : AmyE Front | * Lane3 : AmyE Front | ||
* Lane4 : Duncan | * Lane4 : Bacillus chromosomal DNA (given by Duncan to check) | ||
* Lane5 : ECE112 (plasmid) | * Lane5 : ECE112 (plasmid) | ||
* Lane6 : ECE153 (plasmid) | * Lane6 : ECE153 (plasmid) | ||
Line 52: | Line 52: | ||
- New PCR with 4μL of chromosomal DNA and new PCR program | - New PCR with 4μL of chromosomal DNA and new PCR program | ||
- New gel | |||
* Lane1 : IA751 (chromosomal DNA) | |||
* Lane2 : ECE153 (chromosomal DNA) | |||
* Lane3 : ECE112 (chromosomal DNA) | |||
* Lane4 : ECE112 (plasmid) | |||
* Lane5 : ECE153 (plasmid) | |||
* Lane6 : Bacillus chromosomal DNA (given by Duncan) | |||
* Lane7 : HyperladderI | |||
* Lane8 : agrA | |||
* Lane9 : agrB | |||
*Result | *Result | ||
[[Image:photos09092.gif|300px|center]] | |||
- No insert in IA751 transformed with ECE153 and ECE112 (same size than control!) and however the amylase test is positive for this colony.... | |||
==PCR AmyE again== | ==PCR AmyE again== |
Revision as of 03:43, 16 September 2008
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Chromosomal DNA extraction- ZR soil microbe DNA kit for
- Nanodrop
- Gel
- Only something for IA751, and ECE153 (pure plasmid)??? - New PCR with 4μL of chromosomal DNA and new PCR program - New gel
- No insert in IA751 transformed with ECE153 and ECE112 (same size than control!) and however the amylase test is positive for this colony.... PCR AmyE again- 24μL of SDW, 10μL of MM, 2μL of each primers, 2μL of ECE112
- Very good result for amyE front, nothing for AmyE back |