IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/07/22: Difference between revisions
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(Autocreate 2008/07/22 Entry for IGEM:Cambridge/2008/Notebook/Magnetic_Bacteria) |
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== | ==Transforming TOP10 with mms6 and mamC plasmids== | ||
* Biobricked, E.coli optimised genes mms6 and mamC on plasmids are received from Chris French, Edinburgh. | |||
* Transform TOP10 compotent E.coli with plasmids following the standard TOP10 transformation protocol | |||
* Agar plates with ampicillin 100μg/mL prepared to spread transformant cells onto them - 2 neat and 2 1/10 plates | |||
* Plates incubated overnight | |||
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Revision as of 08:09, 30 July 2008
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Transforming TOP10 with mms6 and mamC plasmids
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