IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/07/22: Difference between revisions
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==Transforming TOP10 with mms6 and mamC plasmids== | ==Transforming TOP10 with mms6 and mamC plasmids== | ||
* Biobricked, E.coli optimised genes mms6 and mamC on plasmids | * Biobricked, E.coli optimised genes mms6 and mamC on plasmids received from Chris French, Edinburgh. | ||
* Transform TOP10 compotent E.coli with plasmids following the standard TOP10 transformation protocol | * Transform TOP10 compotent E.coli with plasmids following the standard TOP10 transformation protocol | ||
* Agar plates with ampicillin 100μg/mL prepared to spread transformant cells onto them - 2 neat and 2 1/10 plates | * Agar plates with ampicillin 100μg/mL prepared to spread transformant cells onto them - 2 neat and 2 1/10 plates | ||
Revision as of 08:09, 30 July 2008
 Magnetic Bacteria
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Transforming TOP10 with mms6 and mamC plasmids
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