IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/07/22: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Yee Yen Tang (talk | contribs) No edit summary |
|||
| (One intermediate revision by one other user not shown) | |||
| Line 1: | Line 1: | ||
<hr width="810px"> | |||
<div id="about"> | |||
{{Cambridge08a}} | |||
</div> | |||
<hr width="810px"> | |||
__NOTOC__ | |||
{|{{table}} width="800" | {|{{table}} width="800" | ||
|- | |- | ||
| Line 7: | Line 13: | ||
==Transforming TOP10 with mms6 and mamC plasmids== | ==Transforming TOP10 with mms6 and mamC plasmids== | ||
* Biobricked, E.coli optimised genes mms6 and mamC on plasmids | * Biobricked, E.coli optimised genes mms6 and mamC on plasmids received from Chris French, Edinburgh. | ||
* Transform TOP10 compotent E.coli with plasmids following the standard TOP10 transformation protocol | * Transform TOP10 compotent E.coli with plasmids following the standard TOP10 transformation protocol | ||
* Agar plates with ampicillin 100μg/mL prepared to spread transformant cells onto them - 2 neat and 2 1/10 plates | * Agar plates with ampicillin 100μg/mL prepared to spread transformant cells onto them - 2 neat and 2 1/10 plates | ||
Revision as of 09:56, 31 July 2008
 Magnetic Bacteria
|
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Transforming TOP10 with mms6 and mamC plasmids
| |
