IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/07/24: Difference between revisions

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__NOTOC__
== Recovery of mms6 and mamC ==
== Recovery of mms6 and mamC ==


Revision as of 02:15, 1 August 2008

Recovery of mms6 and mamC

Recover mms6 and mamC plasmids from colonies:

  • Recover plasmids from single colonies using Zyppy kit following standard protocol
  • Restriction digest with EcoRI and PstI
  • Run on gel and recover mms6 and mamC DNA

Result:

  • Two clear bands obtained on gel at around 400-500 bp
  • Gel cut out and put in eppendorf tubes for gel recovery tomorrow
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