IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/08/04: Difference between revisions
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Lanes of Gel from L to R: | Lanes of Gel from L to R: | ||
[[Image:4aug08.jpg|frame|right|Gel showing bright bands at around 1.4kb on lanes 5-10 corresponding to MagA]] | |||
* Lane 2 - Hyperladder I (5μL DNA) | * Lane 2 - Hyperladder I (5μL DNA) | ||
* Lane 3 - wx (Voltage) | * Lane 3 - wx (Voltage) | ||
Line 39: | Line 39: | ||
* Lane 12 - I0500 (3.9μg/mL DNA detected by nanodrop) | * Lane 12 - I0500 (3.9μg/mL DNA detected by nanodrop) | ||
* Lane 13 - Supercoiled ladder for I0500 plasmid (5μL DNA) | * Lane 13 - Supercoiled ladder for I0500 plasmid (5μL DNA) | ||
Result of Gel: | Result of Gel: |
Revision as of 04:16, 7 August 2008
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MagA RecoveryMagA Colonies and PCR
Gel ElectrophoresisPCR Products and 2 I0500 plasmid samples prepared run of 0.8% agarose gel with EtBr on 90V. Lanes of Gel from L to R:
Result of Gel:
Re-transformation with I0500 to Competent TOP10
Extraction of Yeast GAL1 promoter BBa_J63006
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