IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/08/06: Difference between revisions
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(Autocreate 2008/08/06 Entry for IGEM:Cambridge/2008/Notebook/Magnetic_Bacteria) |
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== | ==Getting Promoter Plasmid and Iron Assay with Fur- Mutants== | ||
=== Iron Assay with Fur- Mutants === | |||
{| border="1" cellpadding="2" | |||
|+Protocol for varying iron concentration | |||
|- | |||
! [Fe]/μM !! 0 !! 4 !! 8 !! 12 !! 16 !! 20 | |||
|- | |||
! Vol. of Fe stock/ml | |||
| 0 || 0.01 || 0.02 || 0.03 || 0.04 || 0.05 | |||
|- | |||
! Vol. of SDW/ml | |||
| 0.50 || 0.49 || 0.48 || 0.47 || 0.46 || 0.45 | |||
|- | |||
! Vol. of fur- cells in M9/ml | |||
| 9.5 || 9.5 || 9.5 || 9.5 || 9.5 || 9.5 | |||
|- | |||
|} | |||
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Revision as of 04:27, 7 August 2008
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Getting Promoter Plasmid and Iron Assay with Fur- MutantsIron Assay with Fur- Mutants
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