IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/08/06

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(Autocreate 2008/08/06 Entry for IGEM:Cambridge/2008/Notebook/Magnetic_Bacteria)
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==Entry title==
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==Getting Promoter Plasmid and Iron Assay with Fur- Mutants==
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=== Iron Assay with Fur- Mutants ===
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{| border="1" cellpadding="2"
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|+Protocol for varying iron concentration
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|-
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! [Fe]/μM !! 0 !! 4 !! 8 !! 12 !! 16 !! 20
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|-
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! Vol. of Fe stock/ml
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| 0 || 0.01 || 0.02 || 0.03 || 0.04 || 0.05
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|-
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! Vol. of SDW/ml
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| 0.50 || 0.49 || 0.48 || 0.47 || 0.46 || 0.45
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! Vol. of fur- cells in M9/ml
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| 9.5 || 9.5 || 9.5 || 9.5 || 9.5 || 9.5
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|}
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Revision as of 06:27, 7 August 2008



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Getting Promoter Plasmid and Iron Assay with Fur- Mutants

Iron Assay with Fur- Mutants

Protocol for varying iron concentration
[Fe]/μM 0 4 8 12 16 20
Vol. of Fe stock/ml 0 0.01 0.02 0.03 0.04 0.05
Vol. of SDW/ml 0.50 0.49 0.48 0.47 0.46 0.45
Vol. of fur- cells in M9/ml 9.5 9.5 9.5 9.5 9.5 9.5




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