IGEM:Cambridge/2008/Notebook/Magnetic Bacteria/2008/08/06: Difference between revisions

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(Autocreate 2008/08/06 Entry for IGEM:Cambridge/2008/Notebook/Magnetic_Bacteria)
 
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==Entry title==
==Getting Promoter Plasmid and Iron Assay with Fur- Mutants==


=== Iron Assay with Fur- Mutants ===


{| border="1" cellpadding="2"
|+Protocol for varying iron concentration
|-
! [Fe]/μM !! 0 !! 4 !! 8 !! 12 !! 16 !! 20
|-
! Vol. of Fe stock/ml
| 0 || 0.01 || 0.02 || 0.03 || 0.04 || 0.05
|-
! Vol. of SDW/ml
| 0.50 || 0.49 || 0.48 || 0.47 || 0.46 || 0.45
|-
! Vol. of fur- cells in M9/ml
| 9.5 || 9.5 || 9.5 || 9.5 || 9.5 || 9.5
|-
|}




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Getting Promoter Plasmid and Iron Assay with Fur- Mutants

Iron Assay with Fur- Mutants

Protocol for varying iron concentration
[Fe]/μM 0 4 8 12 16 20
Vol. of Fe stock/ml 0 0.01 0.02 0.03 0.04 0.05
Vol. of SDW/ml 0.50 0.49 0.48 0.47 0.46 0.45
Vol. of fur- cells in M9/ml 9.5 9.5 9.5 9.5 9.5 9.5




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