IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/07/23: Difference between revisions
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'''2/ Purpose''' : Find the right concentration of antibiotic so that B. subtilis survive | '''2/ Purpose''' : Find the right concentration of antibiotic so that B. subtilis survive | ||
Grow 15uL of B. 1A1 (frozen tube) in 5mL of LB | - Grow 15uL of B. 1A1 (frozen tube) in 5mL of LB | ||
Incubate at 37°C | - Incubate at 37°C | ||
'''3/ Purpose ''': Grow plasmids in TOP10, transformation | '''3/ Purpose ''': Grow plasmids in TOP10, transformation | ||
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1 control : PUC19 | 1 control : PUC19 | ||
Add 20uL of TOP10 competent cells and 0.5uL of Plasmid in an eppendorf | - Add 20uL of TOP10 competent cells and 0.5uL of Plasmid in an eppendorf | ||
30min on ice | - 30min on ice | ||
Heat shock : 60s at 42°C | - Heat shock : 60s at 42°C | ||
2min on ice | - 2min on ice | ||
Add 89.5uL of SOC | - Add 89.5uL of SOC | ||
60min at 37°C | - 60min at 37°C | ||
Put the mix on plate with ampicillin resistance | - Put the mix on plate with ampicillin resistance | ||
- Incubate at 37°C | |||
Revision as of 09:21, 23 July 2008
 Turing Pattern Formation
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Wet work
1/ Purpose Check antibbiotic resistance of our different strains
- Grow 15uL of B. 1A1 (frozen tube) in 5mL of LB - Incubate at 37°C 3/ Purpose : Grow plasmids in TOP10, transformation 4 plasmids :
1 control : PUC19 - Add 20uL of TOP10 competent cells and 0.5uL of Plasmid in an eppendorf - 30min on ice - Heat shock : 60s at 42°C - 2min on ice - Add 89.5uL of SOC - 60min at 37°C - Put the mix on plate with ampicillin resistance - Incubate at 37°C
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