IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/07/23: Difference between revisions

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'''Purpose''' Check antibbiotic resistance of our different strains
'''1/ Purpose''' Check antibbiotic resistance of our different strains


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'''2/ Purpose''' : Find the right concentration of antibiotic so that B. subtilis survive
Grow 15uL of B. 1A1 (frozen tube) in 5mL of LB
Incubate at 37°C
'''3/ Purpose ''': Grow plasmids in TOP10




Revision as of 09:14, 23 July 2008

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Wet work

  • Take one colony from each of the plates grown from the tubes yesterday and plated them again on LA without antibiotics


1/ Purpose Check antibbiotic resistance of our different strains

Strain Plasmid Extracted from Antibiotic use Concentration of antibiotic
DH5alpha PP182 tube Amp 100
MC1061 NONE tube Cm 35
MC1061 PNZ8901 tube Cm 35
DH5alpha PP182 plate Amp 100
MC1061 NONE plate Cm 35
MC1061 PNZ8901 plate Cm 35

2/ Purpose : Find the right concentration of antibiotic so that B. subtilis survive


Grow 15uL of B. 1A1 (frozen tube) in 5mL of LB Incubate at 37°C

3/ Purpose : Grow plasmids in TOP10




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