IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/07/23: Difference between revisions
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'''2/ Purpose''' : Find the right concentration of antibiotic so that B. subtilis survive | '''2/ Purpose''' : Find the right concentration of antibiotic so that B. subtilis survive | ||
Grow 15uL of B. 1A1 (frozen tube) in 5mL of LB | |||
Incubate at 37°C | Incubate at 37°C | ||
'''3/ Purpose ''': Grow plasmids in TOP10 | '''3/ Purpose ''': Grow plasmids in TOP10 | ||
4 plasmids : | |||
* I746000 | |||
* I746100 | |||
* I746101 | |||
* I746001 | |||
1 control : PUC19 | |||
Add 20uL of TOP10 competent cells and 0.5uL of Plasmid in an eppendorf | |||
30min on ice | |||
Heat shock : 60s at 42°C | |||
2min on ice | |||
Add 89.5uL of SOC | |||
60min at 37°C | |||
Put the mix on plate with ampicillin resistance | |||
Icubate at 37°C | |||
Revision as of 09:18, 23 July 2008
 Turing Pattern Formation
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Wet work
1/ Purpose Check antibbiotic resistance of our different strains
Grow 15uL of B. 1A1 (frozen tube) in 5mL of LB Incubate at 37°C 3/ Purpose : Grow plasmids in TOP10 4 plasmids :
1 control : PUC19 Add 20uL of TOP10 competent cells and 0.5uL of Plasmid in an eppendorf 30min on ice Heat shock : 60s at 42°C 2min on ice Add 89.5uL of SOC 60min at 37°C Put the mix on plate with ampicillin resistance Icubate at 37°C
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