IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/07/23: Difference between revisions
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Incubate at 37°C | Incubate at 37°C | ||
'''3/ Purpose ''': Grow plasmids in TOP10 | '''3/ Purpose ''': Grow plasmids in TOP10, transformation | ||
4 plasmids : | 4 plasmids : |
Revision as of 09:19, 23 July 2008
Turing Pattern Formation | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||
Wet work
1/ Purpose Check antibbiotic resistance of our different strains
Grow 15uL of B. 1A1 (frozen tube) in 5mL of LB Incubate at 37°C 3/ Purpose : Grow plasmids in TOP10, transformation 4 plasmids :
1 control : PUC19 Add 20uL of TOP10 competent cells and 0.5uL of Plasmid in an eppendorf 30min on ice Heat shock : 60s at 42°C 2min on ice Add 89.5uL of SOC 60min at 37°C Put the mix on plate with ampicillin resistance Icubate at 37°C
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