IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/07/24: Difference between revisions

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Dilution of antibiotics
Dilution of Amp.
 
{|class="wikitable" style="text-align:center" border="1"
|-
! Concentration (u/ml)
! Dilution
|-
| 100 || 1:1000
|-
| 75 || 3:4000
|-
| 50 || 1:2000
|-
| 25 || 1:4000
|-
| 10 || 1:10000
|-
|}


Dilution of Cm.
{|class="wikitable" style="text-align:center" border="1"
|-
! Concentration (u/ml)
! Dilution
|-
| 35 || 1:1000
|-
| 25 || 3:4000
|-
| 50 || 1:2000
|-
| 25 || 1:4000
|-
| 10 || 1:10000
|-
|}


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Revision as of 09:02, 25 July 2008

Turing Pattern Formation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Results from yesterday

  • Test of antibiotic resistances of strains of last year
Strain Plasmid Extracted from Antibiotic use Observations Conclusion
DH5alpha PP182 tube Amp Many colonies Amp Resistance OK
DH5alpha PP182 plate Amp Many colonies Idem
MC1061 NONE tube Cm Nothing As expecting, no Cm Resist.
MC1061 NONE plate Cm Nothing Idem
MC1061 PNZ8901 plate Cm Maybe a few colonies Contamination??
MC1061 PNZ8901 tube Cm No colonies no Cm Resist.


  • Transformation of E.coli with different plasmids from last year
Strain Inserted plasmid Antibiotic Observation Conclusion
E.coli I746000 Amp No colonies Antibiotic resistance unknown, no Amp Resist., Pb : no terminator
E.coli I746100 Amp No colonies Antibiotic resistance unknown, no Amp Resist., Pb : no terminator
E.coli I746001 Amp Many colonies Transformation OK
E.coli I746101 Amp Many colonies Transformation OK


Wet Work

  • Antibiotic test for Bacillus resistance

We want to find the lowest concentration of antibiotic which kills Bacillus.


Dilution of Amp.

Concentration (u/ml) Dilution
100 1:1000
75 3:4000
50 1:2000
25 1:4000
10 1:10000

Dilution of Cm.

Concentration (u/ml) Dilution
35 1:1000
25 3:4000
50 1:2000
25 1:4000
10 1:10000


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