IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/07/24: Difference between revisions
Yee Yen Tang (talk | contribs) |
Yee Yen Tang (talk | contribs) |
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'''Results''' [Plates were prepared before lunch, at 5pm there were visible growth] | '''Results''' [Plates were prepared before lunch, at 5pm there were visible growth] | ||
Amp Plates: Huge rings of no growth around 100, 75, 50, 25, and 10. | |||
Cm PLates: Tiny rings of no growth around 5, 10, 15 and 25. Small ring of no growth around 35 but not well defined. (No clear zones) | -Amp Plates: Huge rings of no growth around 100, 75, 50, 25, and 10. | ||
-Cm PLates: Tiny rings of no growth around 5, 10, 15 and 25. Small ring of no growth around 35 but not well defined. (No clear zones) | |||
==Single Colony== | ==Single Colony== |
Revision as of 09:28, 25 July 2008
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Results from yesterday
Wet WorkAntibiotic test for Bacillus resistanceWe want to find the lowest concentration of antibiotic which kills Bacillus.
Dilution of Cm. [ 1:1000 means 1 part stock to 1000 part Sterile Distilled Water ]
Results [Plates were prepared before lunch, at 5pm there were visible growth] -Amp Plates: Huge rings of no growth around 100, 75, 50, 25, and 10. -Cm PLates: Tiny rings of no growth around 5, 10, 15 and 25. Small ring of no growth around 35 but not well defined. (No clear zones) Single Colony
Incubate overnight at 37°C Salts for making Bacillus Competent10x Medium A Base
-Aliquot into 5 different bottles. [180mL each] 10x Bacillus Salts
-Aliguot into 5 different bottles. [200mL each] Medium B
-Total volume 200mL NOTE: To complete Medium B, Take 0.2mL of this solution
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