IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/07/25: Difference between revisions

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Line 45: Line 45:
! Concentration (μg/mL) !! 10 !! 7.5 !! 5 !! 2.5 !! 1
! Concentration (μg/mL) !! 10 !! 7.5 !! 5 !! 2.5 !! 1
|-
|-
| 10 mg/mL Stock (from yesterday) || 1:1 || 3:4 || 1:2 || 1:4 || 1:10
| 10 μg/mL Stock (from yesterday) || 1:1 || 3:4 || 1:2 || 1:4 || 1:10
|-
|-
|}
|}
Line 56: Line 56:
! Concentration (μg/mL) !! 35 !! 37.5 !! 40 !! 42.5 !! 45
! Concentration (μg/mL) !! 35 !! 37.5 !! 40 !! 42.5 !! 45
|-
|-
| 35 mg/mL Stock (from yesterday) || 1:1 || 5:6 (from 45) || 1:875 || 17:18 (from 45) || 9:7000
| 35 μg/mL Stock (from yesterday) || 1:1 || 5:6 (from 45μg/mL) || 1:875 || 17:18 (from 45μg/mL) || 9:7000
|-
|-
|}
|}

Revision as of 07:14, 27 July 2008

Turing Pattern Formation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Results from Yesterday

  • Kan resistance of biobricks
Biobrick Antibiotic Contration (μg/mL) Observations Conclusion
I746001 Kan 25 Many colonies Kan Resistance OK
I746101 Kan 25 Many colonies Kan Resistance OK
Control PUC19 Kan 25 No colony No Kan Resistance


  • Antibiotic test

Results for Amp

Even with a concentration of 10μg/mL, Amp kills Bacillus. Since we want to know which is the lowest concentration of Amp which kills B.S, we are going to test with some lower concentrations.

Results for Cm

Even with a concentration of 35μg/mL, ther is not a clear area around the antibiotic disk. So we have to test some higher concentration of Cm.


Wet Work

  • New antibiotic tests

Dilution of Amp

Concentration (μg/mL) 10 7.5 5 2.5 1
10 μg/mL Stock (from yesterday) 1:1 3:4 1:2 1:4 1:10


Dilution of Cm

Concentration (μg/mL) 35 37.5 40 42.5 45
35 μg/mL Stock (from yesterday) 1:1 5:6 (from 45μg/mL) 1:875 17:18 (from 45μg/mL) 9:7000