IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/07/28
 Turing Pattern Formation
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Results from previous days
We wanted to check the size of our biobricks. For I746101, we have a band of about 3000kb, which is the size of the vectorm and a band of about 2000kb. The size of the biobrick is 2057kp, so it should be ok. For I746001, we hqve the same band of 3000kb (the vector) and a band of about 1000kb. The size of the biobrick is 896kb. So, the size of our biobricks are ok.
For Cm, 35μg/mL should be enough to kill B.S. For Amp, Wet Work
We had 2 samples of ppL82 in DH5α in LB solution (~4mL), one from the frozen glycerol tube and one from a colony picked on a plate. Normally plasmid and cells should not be kept in freezer. So, we want to extract this plasmid, check its size and then keep it in the fridge. We do that for the 2 different samples.
- Test the concentration of DNA in each tube
- Digest
- Plate a single colony (from 23/07/08) on a Cm plate - Incubate at 37°C
- Incubate at 37°C
-Pick e single colony of MC1061 (from 22/07/08) and put it in 10mL of LB (to make glycerol stocks) -Incubate at 37°C
- Add 100μL of LB+1A (mix of friday) and 10mL of LB - Incubate at 37°C Prepare Medium A- Add 81mL of Sterile Water, 10mL of 10X Medium A base and 9mL of 10X Bacillus salts
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