IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/07/29: Difference between revisions
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- Cells could be dead : replate them | - Cells could be dead : replate them | ||
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Probable reason'' : we forgot to dilute the medium B, that's why it did not work | ''Probable reason'' : we forgot to dilute the medium B, that's why it did not work | ||
==Wet Work== | ==Wet Work== | ||
Revision as of 07:34, 31 July 2008
 Turing Pattern Formation
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Result from yesterday
The ladder seems to be wrong. So it was really difficult to ckeck the size of our plasmid ppL82. To make, that, we assume that the size of our biobricks was ok, and we estimate the size of our plasmid. The plasmid ppL82 seems to have the right size, but we will have to check again to be able to use a ladder.
We observed B.S. with a microscope. Problem, all B.S. seems to be dead! Possible reasons of this problem : - Problem with the vector (it could be a wrong vector, we are not really sure) - Quantity of DNA, we added 0.5μg. The protocol was 1μg, but normally it should be ok. - B.S. may not be competent (we have to test motility with microscope) - Cells could be dead : replate them
Wet Work | |
