IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/07/30: Difference between revisions
Line 56: | Line 56: | ||
- Plamsid miniprep (for I746001, I746101 and PNZ8901 from big flasks | - Plamsid miniprep (for I746001, I746101 and PNZ8901 from big flasks | ||
- Measure DNA concentration in our samples to decide the volume of DNA we have to add in our preparation | |||
{|class="wikitable" style="text-align:center" border="1" | |||
|- | |||
! | |||
! 260/280 | |||
! DNA concentration (ng/nl) | |||
|- | |||
| I746001 || 1.79 || 50.5 | |||
|- | |||
| I746101 || 1.87 || 54.3 | |||
|- | |||
| PNZ8901 || 1.84 || 87 | |||
|- | |||
|} | |||
- Digest | - Digest | ||
{| class="wikitable" | {| class="wikitable" | ||
Line 81: | Line 97: | ||
|} | |} | ||
Revision as of 08:32, 31 July 2008
Turing Pattern Formation | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Wet Work
Medium A
For this medium, the curb stop to increase logarithmically at 100min.
For this medium, the curb stop to increase logarithmically at 90min.
- Prepare 7 tubes for medium A and 7 tubes for medium A + tryptophan : add 0.45mL of prewarmed medium B and 0.05mL of culture and incubate 90min at 37°C by shaking. - Transform B.S. by adding DNA and incubate 30min at 37°C We have one transformation with ppL82 (add 7.5μL of DNA), one with PNZ8901 (add 10μL of DNA) and one negative control without DNA. - Plate 500μL on Cm plate (35μg/mL)
- Plamsid miniprep (for I746001, I746101 and PNZ8901 from big flasks - Measure DNA concentration in our samples to decide the volume of DNA we have to add in our preparation
|