IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/07/30
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Wet Work
Medium A
For this medium, the curb stop to increase logarithmically at 100min.
For this medium, the curb stop to increase logarithmically at 90min.
- Prepare 7 tubes for medium A and 7 tubes for medium A + tryptophan : add 0.45mL of prewarmed medium B and 0.05mL of culture and incubate 90min at 37°C by shaking. - Transform B.S. by adding DNA and incubate 30min at 37°C We have one transformation with ppL82 (add 7.5μL of DNA), one with PNZ8901 (add 10μL of DNA) and one negative control without DNA. - Plate 500μL on Cm plate (35μg/mL)
- Plamsid miniprep (for I746001, I746101 and PNZ8901 from big flasks - Digest
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