IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/01: Difference between revisions
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The result is good. Each colony of I746001 is about 1000kb (all the same size), for I746101, the size is good (about 2000kb), except for one single colony for which there is nothing. It could be a problem during the manipulation. | The result is good. Each colony of I746001 is about 1000kb (all the same size), for I746101, the size is good (about 2000kb), except for one single colony for which there is nothing. It could be a problem during the manipulation. | ||
* '''Grow received vectors''' | |||
Revision as of 01:17, 6 August 2008
 Turing Pattern Formation
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Wet Work
We had 5 single colonies for each biobrick fro; yestrday. We are going to PCR each single colony. Add 1μL of cells, 7 μL of SDW, 10μL of Phusion Flash master mix, 1μL of VF2 primer and 1μL of VR primer.
Add 5μL of PCR products, 14μL of SDW and 1μL of dye.
The result is good. Each colony of I746001 is about 1000kb (all the same size), for I746101, the size is good (about 2000kb), except for one single colony for which there is nothing. It could be a problem during the manipulation.
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