IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/07: Difference between revisions

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|style="background-color: #EEE"|[[Image:IgemCam.jpg|200px]]<span style="font-size:22px;"> Turing Pattern Formation</span>
|style="background-color: #444444"|[[Image:Signalling_button.gif|200px|center]]
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==Transformation of B.S. IA771==
New transformation with the same protocol than 2 days ago. We will try to add less liquid on plates (to avoid growing colonies in liquid)
{|class="wikitable" style="text-align:center" border="1"
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!  Tube !! Plate 1 !! Plate 2 !! Plate 3 !! Plate 4
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| ECE166 || Cm5 + 200μL of cells || Cm5 + 100μL of cells || Cm5 + 50μL of cells || Cm10 + 100μL of cells 
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| ECE153 || Spc50 + 200μL of cells || Spc50 + 100μL of cells || Spc50 + 50μL of cells ||
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| ECE166 || Cm5 + 100μL of cells || Cm10 + 100μL of cells ||  ||
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| ECE153 || Spc50 + 100μL of cells ||  ||  ||
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| no DNA || Cm5 + 100μL of cells || Cm10 + 100μL of cells || Spc50 + 100μL of cells || 
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| ECE166 (glycerol stock)|| Cm5 + 100μL of cells || Cm10 + 100μL of cells ||  ||
|}
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==Check Vectors==
We want to make a final check for ECE147, 149, 150, 153 and 162.
- Double digest + gel
* Results
- ECE 147, ECE150, ECE153 :ok!
- ECE 149 : 3 bands! not the right vector
- ECE162 : ony one band! It has been uncut... maybe the good vector (according to precedent gel), but not sure
==Test ECE112 transformation==
- for the three different strain (1A1, IA771, IA751), make 12 spots
- take one colony with a loop, and put it on a Cm5 plate and on a Cm5 + Spc100 plate (do that for the 12 spots)
- incubate
==New stock==
- Put ECE171 in 10mL of LB
- Incubate
__NOTOC__

Revision as of 08:17, 5 September 2008

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