IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/07

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Line 4: Line 4:
</div>
</div>
<hr width="810px">
<hr width="810px">
 +
__NOTOC__
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
-
|style="background-color: #444444"|[[Image:Bacillus_button.gif|200px|center]]
+
|style="background-color: #444444"|[[Image:Signalling_button.gif|200px|center]]
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-

Revision as of 06:37, 5 September 2008



Main project page
Previous entry      Next entry

Transformation of B.S. IA771

New transformation with the same protocol than 2 days ago. We will try to add less liquid on plates (to avoid growing colonies in liquid)

Tube Plate 1 Plate 2 Plate 3 Plate 4
ECE166 Cm5 + 200μL of cells Cm5 + 100μL of cells Cm5 + 50μL of cells Cm10 + 100μL of cells
ECE153 Spc50 + 200μL of cells Spc50 + 100μL of cells Spc50 + 50μL of cells
ECE166 Cm5 + 100μL of cells Cm10 + 100μL of cells
ECE153 Spc50 + 100μL of cells
no DNA Cm5 + 100μL of cells Cm10 + 100μL of cells Spc50 + 100μL of cells
ECE166 (glycerol stock) Cm5 + 100μL of cells Cm10 + 100μL of cells

Check Vectors

We want to make a final check for ECE147, 149, 150, 153 and 162.

- Double digest + gel

  • Results

- ECE 147, ECE150, ECE153 :ok!

- ECE 149 : 3 bands! not the right vector

- ECE162 : ony one band! It has been uncut... maybe the good vector (according to precedent gel), but not sure


Test ECE112 transformation

- for the three different strain (1A1, IA771, IA751), make 12 spots

- take one colony with a loop, and put it on a Cm5 plate and on a Cm5 + Spc100 plate (do that for the 12 spots)

- incubate


New stock

- Put ECE171 in 10mL of LB

- Incubate


Personal tools