IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/08: Difference between revisions

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{|{{table}} width="800"
{|{{table}} width="800"
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|style="background-color: #EEE"|[[Image:IgemCam.jpg|200px]]<span style="font-size:22px;"> Turing Pattern Formation</span>
|style="background-color: #444444"|[[Image:Signalling_button.gif|200px|center]]
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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| colspan="2"|
==Result from the transformationof yesterday==
{|class="wikitable" style="text-align:center" border="1"
|-
!  Vector !! Antibiotic !! Quantity of cells added !! number of colonies
|-
| ECE166 || Cm5 || 200μL || 0 : problem!!! 
|-
| ECE166 || Cm5 || 100μL || 2
|-
| ECE166 || Cm5 || 50μL || 1
|-
| ECE166 || Cm10 || 100μL || 0 : no resistance to Cm10!
|-
| ECE166 (spin) || Cm5 || 100μL || a lot, confluent
|-
| ECE166 (spin) || Cm10 || 100μL || 0
|-
| ECE166 (glycerol + spin) || Cm5 || 100μL || about 150, very small
|-
| ECE166 (spin) || Cm10 || 100μL || 0
|-
| ECE153 || Spc50 || 200μL || 21 + a lot of confluent colonies
|-
|  ECE153 || Spc50 || 100μL || 7 + confluent (a few)
|-
|  ECE153 || Spc50 || 50μL || 4
|-
|  ECE153 (spin) || Spc50 || 100μL || 25 + about 200 (small and almost confluent)
|-
|  No DNA || Cm5 || 100μL || 0
|-
|  No DNA || Cm10 || 100μL || 0
|-
|  No DNA || Spc50 || 100μL || about 12, maybe more : problem!!!!!
|}
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|}
==Result for test of ECE112 transformation==
==Control with erythromycin==
==Amylase production screening==
<!-- ## Do not edit below this line unless you know what you are doing. ## -->
__NOTOC__

Revision as of 08:19, 5 September 2008



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