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| |style="background-color: #EEE"|[[Image:IgemCam.jpg|200px]]<span style="font-size:22px;"> Turing Pattern Formation</span> | | |style="background-color: #444444"|[[Image:Signalling_button.gif|200px|center]] |
| |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} |
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| ==Result from the transformationof yesterday==
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| {|class="wikitable" style="text-align:center" border="1"
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| ! Vector !! Antibiotic !! Quantity of cells added !! number of colonies
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| | ECE166 || Cm5 || 200μL || 0 : problem!!!
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| | ECE166 || Cm5 || 100μL || 2
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| | ECE166 || Cm5 || 50μL || 1
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| | ECE166 || Cm10 || 100μL || 0 : no resistance to Cm10!
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| | ECE166 (spin) || Cm5 || 100μL || a lot, confluent
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| | ECE166 (spin) || Cm10 || 100μL || 0
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| | ECE166 (glycerol + spin) || Cm5 || 100μL || about 150, very small
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| | ECE166 (spin) || Cm10 || 100μL || 0
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| | ECE153 || Spc50 || 200μL || 21 + a lot of confluent colonies
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| | ECE153 || Spc50 || 100μL || 7 + confluent (a few)
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| | ECE153 || Spc50 || 50μL || 4
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| | ECE153 (spin) || Spc50 || 100μL || 25 + about 200 (small and almost confluent)
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| | No DNA || Cm5 || 100μL || 0
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| | No DNA || Cm10 || 100μL || 0
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| | No DNA || Spc50 || 100μL || about 12, maybe more : problem!!!!!
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| |}
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| <!-- ## Do not edit below this line unless you know what you are doing. ## -->
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| |}
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| ==Result for test of ECE112 transformation==
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| On Cm5 plates, we have some colonies for each strains, on Cm5 + Spc 100 plates, no colonies on each plates. This result seems good, however, we forgot to make a control. Since, we had some colonies on control plates, it is possible that our bacillus are resistant to Cm5, even if they are not transformed.
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| We addded some control colonies.
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| ==Control with erythromycin==
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| - Prepare erythromycin, stock 5mg/mL : 0.05g in 10mL of SDW
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| - Prepare plates : add 20μL of Ery (5mg/mL) into 200mL of agar (final concentration : 0.5μg/mL)
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| - Do several spots on each plate
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| {|class="wikitable" style="text-align:center" border="1"
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| ! Plate !! Colonies added from
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| |1 || ECE166 + 100μL of cells (2 different plates)
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| |2|| ECE153 + 100μL of cells (2 different plates)
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| |3 || ECE166 (from glycerol stock)
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| |4 || Control (from a plate of IA771 without antibiotic)
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| |5 || IA771 + ECE112 (05/08)
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| |-
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| |6 || IA771 + ECE166 (05/08)
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| |}
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| <!-- ## Do not edit below this line unless you know what you are doing. ## -->
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| ==Amylase production screening==
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| * Prepare agar plate with starch
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| - Starch : 1g of starch in 10mL of SDW
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| - Add 2mL of Starch solution into 200mL of agar, mix
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| -Inoculate on different plates : 1A1 +ECE112 (plate from 05/08), IA751 + ECE112 (plate from 05/08) and IA771 + ECE153
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| <!-- ## Do not edit below this line unless you know what you are doing. ## -->
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| __NOTOC__
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