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| Line 11: |
Line 11: |
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| | colspan="2"| | | | colspan="2"| |
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| ==Result for test of ECE112 transformation==
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| On Cm5 plates, we have some colonies for each strains, on Cm5 + Spc 100 plates, no colonies on each plates. This result seems good, however, we forgot to make a control. Since, we had some colonies on control plates, it is possible that our bacillus are resistant to Cm5, even if they are not transformed.
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| We addded some control colonies.
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| ==Control with erythromycin==
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| - Prepare erythromycin, stock 5mg/mL : 0.05g in 10mL of SDW
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| - Prepare plates : add 20μL of Ery (5mg/mL) into 200mL of agar (final concentration : 0.5μg/mL)
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| - Do several spots on each plate
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| {|class="wikitable" style="text-align:center" border="1"
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| |-
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| ! Plate !! Colonies added from
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| |-
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| |1 || ECE166 + 100μL of cells (2 different plates)
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| |-
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| |2|| ECE153 + 100μL of cells (2 different plates)
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| |-
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| |3 || ECE166 (from glycerol stock)
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| |-
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| |4 || Control (from a plate of IA771 without antibiotic)
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| |-
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| |5 || IA771 + ECE112 (05/08)
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| |-
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| |6 || IA771 + ECE166 (05/08)
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| |}
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| <!-- ## Do not edit below this line unless you know what you are doing. ## -->
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| ==Amylase production screening==
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| * Prepare agar plate with starch
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| - Starch : 1g of starch in 10mL of SDW
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| - Add 2mL of Starch solution into 200mL of agar, mix
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| -Inoculate on different plates : 1A1 +ECE112 (plate from 05/08), IA751 + ECE112 (plate from 05/08) and IA771 + ECE153
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| ==New stocks==
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| - Take 1mL from LB stock oh ECE171 (from 07/08) and put it into 99mL of LB
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| <!-- ## Do not edit below this line unless you know what you are doing. ## -->
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| __NOTOC__
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