IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/08
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Result for test of ECE112 transformationOn Cm5 plates, we have some colonies for each strains, on Cm5 + Spc 100 plates, no colonies on each plates. This result seems good, however, we forgot to make a control. Since, we had some colonies on control plates, it is possible that our bacillus are resistant to Cm5, even if they are not transformed. We addded some control colonies. Control with erythromycin- Prepare erythromycin, stock 5mg/mL : 0.05g in 10mL of SDW - Prepare plates : add 20μL of Ery (5mg/mL) into 200mL of agar (final concentration : 0.5μg/mL) - Do several spots on each plate
Amylase production screening
- Starch : 1g of starch in 10mL of SDW - Add 2mL of Starch solution into 200mL of agar, mix -Inoculate on different plates : 1A1 +ECE112 (plate from 05/08), IA751 + ECE112 (plate from 05/08) and IA771 + ECE153 New stocks- Take 1mL from LB stock oh ECE171 (from 07/08) and put it into 99mL of LB
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