IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/09: Difference between revisions
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*Find out how to do a c-terminal GFP fusion for membrane proteins agrB and agrC | *Find out how to do a c-terminal GFP fusion for membrane proteins agrB and agrC | ||
**Can we fuse GFP and still expect them to localize to the membrane? | **Can we fuse GFP and still expect them to localize to the membrane? | ||
***Seems possible in theory for AgrB. We got lucky because the signal peptide is not on the C terminus, and the C terminus is intracellular. | ***Seems possible in theory for AgrB. We got lucky because the signal peptide is not on the C terminus, and the C terminus is intracellular. http://www.jbc.org/cgi/content/full/277/38/34736#F1 | ||
**Should we do a antibody tag instead? | **Should we do a antibody tag instead? | ||
Revision as of 01:59, 11 August 2008
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Result from Erythromycin testProblem : colonies on each plate
On Monday we will remake the Erythromycin plates, and replate everything with the proper controls. Unfortunately, we do not have a positive control for erythromycin resistance, but if things die (i.e. negative control works), we should be in business. Plans for MondayLab Work
Book/Computer Work
Longer Term Plans
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