IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/09: Difference between revisions
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==Plans for Monday== | ==Plans for Monday== | ||
===Lab Work=== | ===Lab Work=== | ||
*Be sure that the Spc50 plates are working, as the control for Spc50 grew and shouldn't have. | *Be sure that the Spc50 plates are working, as the control for Spc50 grew and shouldn't have. We already know that things can die at Spc100 (the double crossover test from earlier this week) so it is unlikely that the stock is bad. We should test Spc50 again with regular bacillus and with E. coli at 50-100 ug/ml and hope that they die. | ||
*Finish all the agr PCRs, but especially the RBS. | *Finish all the agr PCRs, but especially the RBS. | ||
*Confirm that amyE insertion works, using the amylase/ery tests. | *Confirm that amyE insertion works, using the amylase/ery tests. |
Revision as of 16:46, 10 August 2008
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Result from Erythromycin testProblem : colonies on each plate
On Monday we will remake the Erythromycin plates, and replate everything with the proper controls. Unfortunately, we do not have a positive control for erythromycin resistance, but if things die (i.e. negative control works), we should be in business. Plans for MondayLab Work
Book/Computer Work
Longer Term Plans
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