IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/11: Difference between revisions
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-Nanodrop both DNA | -Nanodrop both DNA | ||
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! Vetor !! 260/280 !! μg/mL | |||
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| ECE171 (10mL) || 1.73 || 136.3 | |||
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| ECE171(100mL) || 1.82 || 99.5 | |||
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- Double digest (EcoRI and PstI) with 10μL of DNA and 1h of incubation | |||
- Samples have been frozen, they should be run onto a gel | |||
==Control of Spc resistance of Bacillus== | |||
- Spc50 plate with transformed IA771 (ECE153, which is Spc resistant) and with IA771 9which should not be Spc resistant) | |||
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Revision as of 09:42, 12 August 2008
 Turing Pattern Formation
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Erytromycin Experiment- New preparation of Ery : 0.05g into 10mL of ETHANOL -We plated again our plates (same than 08/08/08) Amylase screening experiment- New method : add 2g of starch powder into 200mL of Agar, shake, pipette to plate (and avoid bubbles) - Same plates than 08/08/08 Test our stock of ECE171-Plasmid miniprep 9from the samples of 10mL and the one of 100mL) -Nanodrop both DNA
- Double digest (EcoRI and PstI) with 10μL of DNA and 1h of incubation - Samples have been frozen, they should be run onto a gel Control of Spc resistance of Bacillus- Spc50 plate with transformed IA771 (ECE153, which is Spc resistant) and with IA771 9which should not be Spc resistant) | ||||||||||
