IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/13: Difference between revisions
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! Vetor !! 260/280 !! μg/mL !! | ! Vetor !! 260/280 !! μg/mL !! quantity of DNA to add for transformation (μL) | ||
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| ECE153 || 1.5 || 18.4 || 27.2 | | ECE153 || 1.5 || 18.4 || 27.2 | ||
Revision as of 01:35, 15 August 2008
 Turing Pattern Formation
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Results for Starch plates- Add 5mL of iodine - 1A1 or IA751 : big zones of clearance - IA751 + ECE112 : no zones of clearance (photos), just small white points on colonies : the gene AmyE seems to be knocked out, transformation ok! - 1A1 + ECE112 : problem, soft agar melted... impossible to observe!
Transformation- We used IA751 plate from 12/08/08, vectors ECE 153, 166, 171 - Spectrophotometer : blank made with medium A
- t0 = 120min - Follow the protocol of transformation - Plasmid miniprep of ECE153, 166, 171 (to make the transformation) - Nanodrop ( to add 0.5μg of DNA for transformation)
- Plate (with appropriate antibiotics)
Check our stock of ECE171- We made big stocks of ECE171, before keeping it, we wanted to check it, so we run double digest on a gel!! - Results : problem!! - 2 possible causes : Double digest was made " days before and kept in the freezer (possible degradation) + too much DNA on the gel - New double digest (from pellets in the freezer) + new gel - Results : ok! == | |||||||||||||||||||||||||||||||||
