IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/14: Difference between revisions
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== Results of transformation== | == Results of transformation== | ||
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! Vector !! Antibiotic !! number of colonies | |||
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| ECE166 || Cm5 || 31 | |||
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| Control || Cm5 || 0 | |||
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| ECE171 || Kan5 || 5 + a lot of small colonies | |||
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| Control || Kan5 || 100μL || 1 + 5 very small | |||
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| ECE153 || Spc50 || about 25 (on a side) | |||
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| Control || Spc50 || 6 + 2 very small | |||
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- Problem with our control, maybe we should check the resistance of competent cells (before transformation) | |||
==BioBrick extraction for testing promoters and RBS in ''B.subtillis''== | ==BioBrick extraction for testing promoters and RBS in ''B.subtillis''== |
Revision as of 01:56, 15 August 2008
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Results of transformation
- Problem with our control, maybe we should check the resistance of competent cells (before transformation) BioBrick extraction for testing promoters and RBS in B.subtillisThe primers for inducible B.subtillis promoters have been ordered. Meanwhile, we would like to be able to compare the RBS and promoter strengths in E.coli and B.subtillis, using GFP fluorescence to quantify gene expression. We attempted to isolate 4 BioBricks: I13522 (GFP under constitutive promoter), E0040 (GFP only), R0040 (a promoter), & B0034 (an E.coli RBS). So far, only the former two, extracted from 2007 wells, grew after transformation. Single-colony PCR was used to test the transformants. Expected VF2-VR fragment sizes: I13522 - ~2.4 kb E0040 - 958 bp R0040 - 292 bp B0034 - 250 bp
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