IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/14: Difference between revisions
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(Autocreate 2008/08/14 Entry for IGEM:Cambridge/2008/Notebook/Turing_Pattern_Formation) |
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== | ==BioBrick extraction for testing promoters and RBS in ''B.subtillis''== | ||
The primers for inducible ''B.subtillis'' promoters have been ordered. Meanwhile, we would like to be able to compare the RBS and promoter strengths in ''E.coli'' and ''B.subtillis'', using GFP fluorescence to quantify gene expression. | |||
We attempted to isolate 4 BioBricks: I13522 (GFP under constitutive promoter), E0040 (GFP only), R0040 (a promoter), & B0034 (an ''E.coli'' RBS). | |||
So far, only the former two, extracted from 2007 wells, grew after transformation. Single-colony PCR was used to test plasmids. | |||
Expected VF2-VR fragment sizes: | |||
I13522 - ~2.4 kb | |||
E0040 - 958 bp | |||
R0040 - 292 bp | |||
B0034 - 250 bp | |||
Revision as of 16:01, 14 August 2008
 Turing Pattern Formation
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BioBrick extraction for testing promoters and RBS in B.subtillisThe primers for inducible B.subtillis promoters have been ordered. Meanwhile, we would like to be able to compare the RBS and promoter strengths in E.coli and B.subtillis, using GFP fluorescence to quantify gene expression. We attempted to isolate 4 BioBricks: I13522 (GFP under constitutive promoter), E0040 (GFP only), R0040 (a promoter), & B0034 (an E.coli RBS). So far, only the former two, extracted from 2007 wells, grew after transformation. Single-colony PCR was used to test plasmids. Expected VF2-VR fragment sizes: I13522 - ~2.4 kb E0040 - 958 bp R0040 - 292 bp B0034 - 250 bp
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