IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/14

From OpenWetWare
Jump to navigationJump to search
Turing Pattern Formation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Results of transformation

Vector Antibiotic number of colonies
ECE166 Cm5 31
Control Cm5 0
ECE171 Kan5 5 + a lot of small colonies
Control Kan5 1 + 5 very small
ECE153 Spc50 about 25 (on a side)
Control Spc50 6 + 2 very small

- Problem with our control, maybe we should check the resistance of competent cells (before transformation)

BioBrick extraction for testing promoters and RBS in B.subtillis

The primers for inducible B.subtillis promoters have been ordered. Meanwhile, we would like to be able to compare the RBS and promoter strengths in E.coli and B.subtillis, using GFP fluorescence to quantify gene expression.

We attempted to isolate 4 BioBricks: I13522 (GFP under constitutive promoter), E0040 (GFP only), R0040 (a promoter), & B0034 (an E.coli RBS). So far, only the former two, extracted from 2007 wells, grew after transformation. Single-colony PCR was used to test the transformants.

Expected VF2-VR fragment sizes:

I13522 - ~2.4 kb E0040 - 958 bp R0040 - 292 bp B0034 - 250 bp

Transformation of vectors 188, 189, 190

- We already tried yes


Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Cambridge/2008/Notebook/Turing_Pattern_Formation/2008/08/14&oldid=231594"