IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/15

From OpenWetWare
Jump to navigationJump to search
Turing Pattern Formation <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Transformation ECE188, 189, 190 into E.coli

- Results : only our control plate grew!!

- Maybe we did not add enough DNA, or there is another problem. We will e mail the guy from Bacillus center before trying again, because we don't have enough DNA.

Lab work

  • Plasmid miniprep ECE 151 (x2), ECE 153, ECE 166
  • Nanodrop results :
260/280 ng/μL
ECE 151 (3) 1.79 86.8
ECE 151 1.88 106.3
ECE 153 1.80 32.2
ECE 166 1.87 36.2


  • Xylose Induction with ECE 153

- 4 Tubes


  • Glycerol Stock Transformation

- Spin down cells (competent) from 5/8 and 13/8 stocks

- Remove liquid, resuspend in Medium B

- Incubate for 60 mins at 37°C

- Add ECE 166 into tubes

- Incubate for 30 mins at 37°C

- Plate out on CM 5 plates

- 4 plates

= 771 (5/8) + ECE 166

= 771 (5/8) Control

= 771 (13/8) + ECE 166

= 771 (13/8) Control


  • Transforming ECE 188, 189, 190 (3rd try)

- 2 tubes of chemically competent top 10

- Spin down, remove liquid

- Resuspend cells in 100μL of CaCl2 50mM

- 4 tubes with 50μL of cells

= ECE 190 (all)

= ECE 189 (all)

= ECE 188 (all)

= PUC 9 (5μL) [ Control ]

- Ice for 30 mins

- 42°C for 2 mins

- Ice for 2 mins

- Incubate at 37°C for 2 hours

Plate out on Amp 100 (Neat)




Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Cambridge/2008/Notebook/Turing_Pattern_Formation/2008/08/15&oldid=233003"