IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/16

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Result from the days before

  • For Ery plates

- Everything grew, we will try again to see if there is a problem with the antibiotic (and we will do a negative control with the strain IA751)

  • Transformation of E.coli with ECE188, 189, 190

- We have a result from our 3 transformation in E.coli. To transform E.coli with these vectors we have to wait for 2 days before seeing cells!! We will check the vectors next week.

  • Transformation of B.S. IA771 (glycerol stocks)

- Nothing! Problem with the storage of competent IA771???


Agr A and C ligation to pSB4C5

Ligation was performed using Fermentas Rapid Ligation kit according to their protocol.

After ligation, samples were visualized on a gel, but little product of the correct size was seen.

For agrA we should have gotten a band of size 3700 (3000plasmid + 700 insert)

For agrC we should have gotten a band of size 4300 (3000plasmid + 1300insert)


The bands of appropriate sizes were extracted for transformation.




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