IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/16

From OpenWetWare
Jump to navigationJump to search
<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>


Agr A and C ligation to pSB4C5

Ligation was performed using Fermentas Rapid Ligation kit according to their protocol.

After ligation, samples were visualized on a gel, but little product of the correct size was seen.

For agrA we should have gotten a band of size 3700 (3000plasmid + 700 insert)

For agrC we should have gotten a band of size 4300 (3000plasmid + 1300insert)


The bands of appropriate sizes were extracted for transformation.




Retrieved from "https://openwetware.org/mediawiki/index.php?title=IGEM:Cambridge/2008/Notebook/Turing_Pattern_Formation/2008/08/16&oldid=238631"