IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/18: Difference between revisions
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(Autocreate 2008/08/18 Entry for IGEM:Cambridge/2008/Notebook/Turing_Pattern_Formation) |
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== | ==Check promoters== | ||
On Friday, PCR out promoters, we want to check them. | |||
- Run a gel | |||
- Results : good size of bands!!! | |||
==Test Ery efficiency== | |||
Since all Ery plates grew, we want to check the antibiotic stock. | |||
- New Ery plate of IA751 (they should die) | |||
- New LB stock (Ery5) with IA751 | |||
==Single colony plates== | |||
- New plates (Cm5 + Amp100) with single colony of ECE188 (from 14/08), ECE189 (from 13/08) and ECE190 (from 14/08) | |||
- Grow the same single colony into LB with antibiotic | |||
Revision as of 09:03, 18 August 2008
 Turing Pattern Formation
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Check promotersOn Friday, PCR out promoters, we want to check them. - Run a gel - Results : good size of bands!!! Test Ery efficiencySince all Ery plates grew, we want to check the antibiotic stock. - New Ery plate of IA751 (they should die) - New LB stock (Ery5) with IA751 Single colony plates- New plates (Cm5 + Amp100) with single colony of ECE188 (from 14/08), ECE189 (from 13/08) and ECE190 (from 14/08) - Grow the same single colony into LB with antibiotic
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