IGEM:Cambridge/2008/Notebook/Turing Pattern Formation/2008/08/18: Difference between revisions

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- Results : good size of bands!!!
- Results : good size of bands!!!
==Test Ery efficiency==
Since all Ery plates grew, we want to check the antibiotic stock.
- New Ery plate of IA751 (they should die)
- New LB stock (Ery5) with IA751
==Single colony plates==
- New plates (Cm5 + Amp100) with single colony of ECE188 (from 14/08), ECE189 (from 13/08) and ECE190 (from 14/08)
- Grow the same single colony into LB with antibiotic
==Transformation of glycerol stocks of B.S.==
The result from the last transformation of glycerol stocks was negative> We tried again with two different protocols.
* '''First protocol'''
- Spin the glycerol stock, throw out the supernatant (just keep about 100μL), add 0.5μL of Medium B
- Incubate about 1h15
- Add 0.6μg of ECE166 (because we managed to transform BS with this vector)
- Incubate 1h30
- Plate on Cm5 plates (DNA less control and transformed cells)
* '''Second protocol'''
- Spin the glycerol stock, throw out the supernatant (just keep about 100μL), add 0.5μL of Medium B
- Add 0.6μg of ECE166 (because we managed to transform BS with this vector)
- Incubate 30min
- Plate on Cm5 plates (transformed cells)
- Plate cells after 10min of incubation (without DNA) on blank plates (to see if they are alive)
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==Transformation of AgrA and AgrC==
==Transformation of AgrA and AgrC==

Revision as of 08:45, 5 September 2008

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Check promoters

On Friday, PCR out promoters, we want to check them.

- Run a gel

- Results : good size of bands!!!

Transformation of AgrA and AgrC

AgrA and C gell-extracted ligation was transformed into Top 10 cells using standard protocol. Puc9 was used as a positive control.


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